FIG 3.
Effects of mortalin depletion on MEK/ERK activation and PP1α/PP2A expression in B-RafV600E and K-RasG12C/D tumor cells. Total cell lysates were analyzed by Western blotting. (A) SK-MEL 28 cells were infected with two different lentiviral shRNA systems targeting mortalin (shMort#1 and shMort#2) for the indicated periods before harvest. pLL3.7 was the control virus. (B) A375 cells stably expressing doxycycline-inducible pTRIPZ shRNA targeting mortalin (shMort-mir) were treated with doxycycline for 5 days. (C and D) MiaPaCa-2 (C) and PANC-1 (D) cells were infected with shMort#1 or shMort#2 for 6 days. pPP1 indicates phosphorylation at Thr320 of PP1.