FIG 6.

Mortalin depletion decreases PP1α-MEK1/2 interaction in SK-MEL 28 cells. (A) Total lysates of SK-MEL 28 cells infected with shMort for 4 days were subject to IP using PP1α-specific antibody. Co-IP of the indicated proteins was determined by Western blotting. (B) Endogenous MEK2 was pulled down using an anti-MEK2 antibody. After three stringent washes with the IP buffer, MEK2 IP fractions were incubated with 0.5 μM recombinant PP1α for 60 min before Western blotting. GST was used as the control for recombinant PP1α.