FIG 8.

The PBD mutant facilitates PP1α-MEK2 interaction less efficiently than full-length mortalin. (A and B) Dose-dependent effects of mortalin on MEK2-PP1α interaction. Recombinant MEK2 and PP1α (0.5 μM) were incubated for 60 min in the presence of different doses of GST-mortalin (A) or GST-PBD (B) before IP using anti-MEK2 antibody and protein G-agarose. (C) The degree of PP1α-MEK2 interaction affected by GST-mortalin or GST-PBD was determined by densitometry of the PP1α signals normalized by MEK2 signals in panels A and B.