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Effects of adenosine nucleotides on mortalin interactions with PP1α and MEK2. GST-mortalin (A and B) or GST-PBD (C and D) (0.5 μM) was incubated for 60 min with 0.5 μM recombinant PP1α (A and C) or MEK2 (B and D) in the presence of 1 μM ATP or ADP before GST pulldown using glutathione-Sepharose and Western blotting.
