FIG 1.

JHDM1D-AS1 is coexpressed with JHDM1D under nutrient starvation. (A) JHDM1D-AS1 and JHDM1D share a promoter at chr 7. The histone H3K27ac marks and open chromatin region comprise the shared promoter. FAIRE-seq, H3K27Ac ChIP-seq, and RNA seq were conducted in PANC-1 cells under nutrient starvation (NS) in comparison to the nutrient-rich control (CON) conditions. (B) JHDM1D-AS1 RNA expression levels are highly correlated with JHDM1D levels in various cancer cell lines (the expression data were obtained from Affymetrix Exon array data obtained from our institutional database, RefExA [http://www.lsbm.org/site_e/database/index.html]). Pearson's correlation test was used (P < 0.05 for significance; r = correlation coefficient). (C) CRISPR/Cas-mediated genomic deletion of the JHDM1D-AS1 promoter region downregulates the expression of both JHDM1D-AS1 and JHDM1D. A schematic of the genomic target regions is shown on the left. (D) The expression level of JHDM1D is increased in response to nutrient starvation in PANC-1, AsPC-1, HeLa, T98G, and SW620 cells. (E) The expression level of JHDM1D-AS1 is increased in response to nutrient starvation in PANC-1, AsPC-1, HeLa, T98G, and SW620 cells. (F) The expression level of JHDM1D is increased in response to nutrient starvation in fibroblastic NHDFs and endothelial HUVECs. (G) The expression level of JHDM1D-AS1 is increased in response to nutrient starvation in NHDFs and HUVECs. (H) The expression levels of JHDM1D and JHDM1D-AS1 are increased in the avascular tumor tissues from day 3 to day 5. Data are presented as the mean ± standard error of the mean (SEM) from at least three independent experiments. The expression of each transcript is reported relative to that of β-actin and was determined by real-time quantitative PCR (qPCR) analysis. Student's t tests were performed for the indicated comparisons (***, P < 0.005; §§§, P < 0.005).