FIG 2.

JHDM1D-AS1 is localized in both the cytoplasm and nucleus and increases cell proliferation in vitro. (A) Subcellular localization of JHDM1D-AS1. PANC-1 and AsPC-1 cells were subjected to subcellular fractionation, and the amounts of JHDM1D-AS1 in each fraction were evaluated by quantitative real-time PCR. ACTB, BIRC5, S14, and GAPDH genes were used as cytoplasm and nuclear marker genes. MALAT1, Xist, and TUG1 were used as nuclear markers. (B) Expression of JHDM1D-AS1 is stably induced by retroviral transduction of JHDM1D-AS1 in PANC-1 and AsPC-1 cells. (C) The expression level of JHDM1D is not affected by JHDM1D-AS1 overexpression in PANC-1 and AsPC-1 cells. (D) Subcellular localization of JHDM1D-AS1 is not altered by JHDM1D-AS1 overexpression. (E) JHDM1D-AS1 overexpression slightly increased cell growth in control medium in PANC-1 and AsPC-1 cells. (F) JHDM1D-AS1 overexpression does not affect cell growth in nutrient starvation medium in PANC-1 and AsPC-1 cells. Data are presented as the mean ± SEM from at least three independent experiments. The expression of each transcript is reported relative to that of β-actin and was determined by real-time qPCR analysis. Student's t tests were performed for the indicated comparisons (***, P < 0.005).