FIG 6.

JHDM1D-AS1 overexpression triggers inflammation and upregulation of genes for proinflammatory factors such as MMP3, MMP9, S100a8, and S100a9 in host-derived cells in tumor tissues. (A) Heat map representation of 1,559 mouse genes that were up- or downregulated by more than 2-fold in tumor tissues derived from JHDM1D-AS1-overexpressing AsPC-1 cells in vivo. (B) Mouse genes related to tumor necrosis factor alpha (TNF-α) signaling via NF-κB are upregulated in tumor tissues of mice inoculated with JHDM1D-AS1-expressing AsPC-1 cells. (C) Expression of genes for mouse proinflammatory factors such as mMmp3, mMmp9, mS100a8, and mS100a9 is increased in tumor tissue derived from JHDM1D-AS1-expressing PANC-1 and AsPC-1 cells. Data are presented as the mean ± SEM from at least three independent experiments. The expression of each transcript is reported relative to that of β-actin and was determined by real-time qPCR analysis. Student's t tests were performed for the indicated comparisons (***, P < 0.005).