Figure 2.

OPC Differentiation Is affected during Remyelination in Pdgfb^ret/ret Mice

(A and B) Illustrations of decreased PC coverage in Pdgfb^ret/ret mice (A) and of lysolecithin-induced demyelination (B).

(C and J) Mature oligodendrocytes (Olig2+/APC+) and OPCs (Olig2+/APC−) in lesions of control and Pdgfb^ret/ret mice at 14 dpl (C) and 21dpl (J).

(D–G and K–N) Quantitative analyses of oligodendroglia at 14 dpl (D–G) and 21 dpl (K–N).

(H and I) In situ hybridization and quantification for PLP of control and Pdgfb^ret/ret mice at 14 dpl.

(O and P) Toluidine blue staining of remyelination in control and Pdgfb^ret/ret mice at 21 dpl (O) and its quantification by relative ranking analysis (P).

Means ± SEM are shown. Data were analyzed by Student’s t test or Mann-Whitney U test. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Scale bars, 100 μm.