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. 2017 Aug 22;20(8):1755–1764. doi: 10.1016/j.celrep.2017.08.007

Figure 3.

Figure 3

PCs Promote OPC Differentiation and Accelerate Remyelination in Demyelinated Organotypic Slices

(A) Schematic of the experimental design.

(B–E) Fluorescence images (B) displaying CNP+/Olig2+, MBP+/Olig2+, and NG2+/Olig2+ oligodendrocytes at 4 DIV and their respective graphs (C–E).

(F and G) PC-CM increases the generation of oligodendrocytes from OPCs. Graphs display (F) the OPC survival rate (NG2+/Caspase+) and (G) the OPC proliferation rate (NG2+/Ki67+) under different conditions.

(H) Illustration of lysolecithin-induced demyelination of slice cultures.

(I) Images showing axons (NFH+) and myelin (MBP+) of remyelinating cerebellar slices exposed to control medium versus PC-CM at 4, 6, and 8 days post lysolecithin treatment.

(J) Quantification of the MBP/NFH ratio shows that PC-CM increases the remyelination rate. 3 or more independent OPC and slice preparations were used.

Means ± SEM are shown. Data were analyzed by two-way ANOVA followed by Bonferroni’s post hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ∗ indicates statistical difference for PC-CM and # for MSC-CM. Scale bars, (B) 100 μm and (I) 50 μm.