Table 1.
Mean number (± standard error) of follicular apoptotic cells and comparative statistics between wPip+ and wPip- groups
| Period of oogenesis | Mean no. of apoptotic follicular cells per ovary (1) | Mean no. of primary follicles that have at least 1 apoptotic event (2) | Mean no. of secondary follicles that have at least 1 apoptotic event (3) | Statisticsb | |
|---|---|---|---|---|---|
| PVP | |||||
| Day 1 | wPip+ | 3.3 ± 3.29 | nd | nd | (1) t = 0.6285, P = 0.5334c |
| wPip- | 2.55 ± 4.2 | nd | nd | ||
| Day 2a | wPip+ | 12.85 ± 13.99** | nd | 9.65 ± 5.83** | (1) U = 57.5, P < 0.0001; (3) t = 4.274, P < 0.0001 |
| wPip- | 3.45 ± 3.52** | nd | 3.15 ± 3.5** | ||
| Day 3 | wPip+ | 8.4 ± 5.25 | 0.4 ± 0.82** | 7.55 ± 4.26 | (1) t = 0.2246, P = 0.8081; (2) U = 81.5, P = 0.0013; (3) t = 0.9477, P = 0.3493 |
| wPip- | 8.8 ± 5.09 | 1.9 ± 1.8** | 6.3 ± 4.08 | ||
| Day 4 | wPip+ | 4.43 ± 4.02 | 0.15 ± 0.37 | 3.6 ± 3.36 | (1) t = 1.724, P = 0.0929; (2) U = 190, P = 0.7890; (3) t = 1.633, P = 0.1107 |
| wPip- | 2.5 ± 2.7 | 0.2 ± 0.41 | 2.1 ± 2.36 | ||
| Day 5 | wPip+ | 2.05 ± 2.26* | 0.4 ± 0.82 | 1.35 ± 1.53* | (1) t = 2.106, P = 0.0418; (2) U = 145.5, P = 0.1382; (3) t = 2.088, P = 0.0435 |
| wPip- | 3.7 ± 2.68* | 1 ± 1.34 | 2.55 ± 2.06* | ||
| VP | |||||
| 6 h | wPip+ | 9.15 ± 3.67** | 1.2 ± 1.58* | 7.2 ± 2.76** | (1) t = 4.254, P < 0.0001; (2) t = 2.401, P = 0.0214; (3) t = 3.484, P = 0.0013 |
| wPip- | 3.75 ± 4.33** | 0.3 ± 0.57* | 3.4 ± 4.02** | ||
| 12 h | wPip+ | 7.95 ± 8.91** | 0.7 ± 0.86 | 6.4 ± 7.05** | (1) t = 2.543, P = 0.0152; (2) U = 172.5, P = 0.4569; (3) t = 3.015, P = 0.0046 |
| wPip- | 2.3 ± 3.03** | 0.7 ± 1.34 | 1.5 ± 1.76** | ||
| 24 h | wPip+ | 6.25 ± 14.57** | 2.05 ± 3.69** | 1.1 ± 1.16** | (1) U = 80.5, P < 0.0012; (2) U = 110, P = 0.0114; (3) U = 110, P = 0.0139 |
| wPip- | 0.75 ± 1.45** | 0.4 ± 0.94** | 0.3 ± 0.66** | ||
Abbreviations: PVP pre-vitellogenic period, VP vitellogenic period, wPip+ infected, wPip- uninfected; nd, no data, T student t test, MW Mann-Whitney U- test, P P-value
*P < 0.05
**P < 0.02
aUndifferentiated follicles, considered as secondary in the table
b(1) statistics related to mean number of apoptotic follicular cells per ovary; (2) statistics related to mean number of primary follicles that have at least 1 apoptotic event; (3) statistics related to mean number of secondary follicles that have at least 1 apoptotic event
cAll values for degrees of freedom for Student’s t - test: df = 38