Fig. 2.

Structure and post-translational modifications of TRIM28 protein. RING – Really Interesting New Gene; zinc finger type domain which contains a C₃HC₄ amino acid motif that binds two zinc cations; has an intrinsic E3 Ubiquitin ligase activity; B1, B2 – B-box type 1 and B-box type zinc finger type domains of around 40 amino acid residues in length; CC – Coiled Coil, a structural motif in which 2 alpha-helices are coiled together (like the strands of a rope); RBCC – RING domain followed by B-boxes and CC domain, RBCC domain is responsible for interaction with KRAB domain of KRAB-ZFPs; TSS – TRIM Specific Sequence; HP1BD – HP1 protein binding domain, contains a consensus PxVxL motif; PHD – Plant Homeodomain, zinc finger type domain which contains a C₄HC₃ amino acid motif that binds two zinc cations, has an intrinsic E3 SUMO ligase activity; BROMO – Bromodomain, together with PHD are responsible for NuRD/SETDB1 recruitment and binding