Figure 1.

Effect of lipopolysaccharide (LPS; 400 ng/kg; iv.) and acetylcholinesterase inhibitors: Donepezil (2.5 mg/animal; iv.) and Neostigmine (0.5 mg/animal; iv.) injections on blood concentration of luteinising hormone (LH) (a) and follicle-stimulating hormone (FSH) (b) concentration in the blood plasma. The data are presented as the mean value ± SEM. All experiments consisted of a baseline period when no treatment was given (2 to 0.5 h before) and a period after treatment (1 to 3 h after). ∗: asterisk indicates statistically significant differences between the period when no treatment was given and a period after treatment according to Student's t-test for dependent samples (“repeated measures”). The results of blood hormones concentration obtained only after treatment period were analysed using a two-way ANOVA. Different capital letters indicate significant differences according to a two-way ANOVA followed by Fisher's post hoc test. Statistical significance was defined as P < 0.05.