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Thermodynamic parameters of the binding of cytochrome c with WT Aio and AioB-F108A.
| Aio | kd (μM) | ΔH (kcal mol− 1) | ΔS (cal mol− 1 deg− 1) | Stoichiometry |
|---|---|---|---|---|
| WT | 2.3 ± 0.7 | 6.0 ± 0.1 | 46.1 ± 1.1 | 0.85 ± 0.15 |
| AioB-F108A | 7.8 ± 1.7 | 3.0 ± 0.3 | 33.5 ± 1.1 | 0.82 ± 0.23 |
Results are the averages and standard deviations of three experiments with three separate enzyme preparations.
