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. 2016 Apr 26;15:67. doi: 10.1186/s12934-016-0465-z

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© Papapetridis et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Theoretical stoichiometric comparison of the anaerobic metabolism of acetate reducing S. cerevisiae expressing a strictly NAD⁺-dependent 6-PGDH to acetate reducing S. cerevisiae expressing the native 6-PGDH. Numbers in boxes represent the carbon distribution and grey numbers in boxes represent the requirement for glucose and cofactors in mmol g⁻¹_x, normalized for the formation of 1 g of biomass in the two scenarios: native, NADP⁺-dependent 6-PGDH (top, blue colour) and heterologous, NAD⁺-dependent 6-PGDH (bottom, red colour). Blue glycolysis and alcoholic fermentation; Green pentose-phosphate pathway; Purple glycerol formation pathway; Black acetate to ethanol reduction pathway. Grey biosynthesis according to [68], which, together with the ATP requirement for biosynthesis, was assumed to be identical for both scenarios. Glycerol formation in this case was assumed to be zero. The Acs-catalysed reaction requires the hydrolysis of ATP to AMP and pyrophosphate, which is stoichiometrically equivalent to hydrolysis of 2 ATP to 2 ADP. The oxidative branch of the pentose phosphate pathway was assumed to be the only NADPH formation pathway. Figure adapted from [21]