Figure 2.

Culture of human primary monocyte–derived macrophages and THP-1 macrophages on TRPs and CTRL and their detachment. (a) Representative microscopic images of adherent macrophages differentiated for 6 days on TRPs and CTRL are shown in the first (primary monocyte–derived macrophages) and third (THP-1) columns. Representative microscopic images of the TRPs after detaching macrophages for 60 min at 25°C are shown in the second (primary monocyte–derived macrophages) and fourth (THP-1) columns. Magnification: 40×. (b) Quantification of detached macrophages from TRPs by temperature reduction compared to trypsin-treated macrophages from CTRL. The means and standard deviations of three independent experiments are shown.