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. 2017 Aug 25;8:2041731417726428. doi: 10.1177/2041731417726428

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Metabolic activity and vitality state of macrophages attached to TRPs and CTRL (a) and detached (b) from TRPs and CTRL. (a) MTT assay of primary monocyte–derived macrophages (left panel) and THP-1 macrophages (right panel). Data obtained were set into relation to data from macrophages on CTRL. (b) Viability analysis of detached primary monocyte–derived macrophages (left panel) and THP-1 macrophages (right panel) were performed by flow cytometry. Cells were stained with 7-AAD (dead cells) and Annexin V PE (apoptotic cells) allowing discrimination of vital, apoptotic, and dead cells. For better view, error bars are only shown in one direction. For MTT and vitality assays, the means and standard deviations of three independent experiments are shown. (b) Asterisks inside the bar represent significant changes in vital cells detached from TRPs compared to detached cells from CTRL. Asterisks above the bars represent significant changes of dead cells from TRPs compared to CTRL.