Supplementary Figure 2.

Gating strategy for flow cytometry analysis of phagocytosis of macrophages: Two upper panels: primary monocyte–derived macrophages, two lower panels: THP-1 macrophages. Representative analyses of macrophages grown on CTRL, incubated with or without opsonized pHrodoTM BioParticles for 24 h and detached by trypsin are shown. Gating for macrophage populations and their representative histogram plot for pHrodoTM MFI is shown.