Figure 1.

(a–d) H&E and (e–h) Movat’s pentachrome staining highlighting the effects of decellularization by SDS (b, f), trypsin (c, g), and Triton X-100 (d, h) compared to native tissue (a, e). All three methods show effective removal of cellular and nuclear material. SDS slides show preservation of leaflet structure and ECM components. Trypsin slides show a “loosening” of the ECM network and loss of structural proteins. Triton X-100 slides show good preservation of leaflet structure but loss of GAGs from the ECM.

Source: Figure reprinted from Liao et al.¹⁴ with permission. Copyright 2008 Elsevier: Biomaterials.