Figure 3.
Immunological staining demonstrating successful recellularization of leaflets from pulmonary valves conjugated with CD133 and implanted in the pulmonary position in sheep. Texas Red–labeled secondary antibodies show αSMA (top row) and vimentin (bottom row), and the nuclei are DAPI counterstained. Percentage values are the percent of cells with positive expression compared to the total number of cells which represent the mean calculated from all three leaflets. Note the high αSMA expression in the tissue-engineered leaflets compared to native leaflets. L denotes lumen. * indicates p < 0.05. Scale bars are 100 µm.
Source: Figure reprinted from Williams et al.96 with permission. Copyright 2015 Springer Science: Journal of Cardiovascular Translational Research.