Figure 2.

mIMCD3 shPkd1 cyst growth can be enhanced with forskolin, and this increase can be prevented with positive control compounds rapamycin, sorafenib, roscovitine, and NVP-BEZ-235. (A) High-resolution images obtained from an unexposed (0.2% DMSO, top panel) and a forskolin-treated (bottom panel) cyst. (B) Using Ominer analysis tools, cyst size could be expressed as a function of forskolin concentration. Data points represent means ± SD of eight replicate wells (technical replicates). (C) Representative images of control compounds after coexposure with forskolin, including unstimulated (0.2% DMSO) control. (D) Quantification of average cyst size from C. Whiskers represent min to max. Rapamycin, roscovitine, sorafenib, and NVP-BEZ-235 tested in quadruplicate (technical replicates), unstimulated (n = 16 replicate wells) and stimulated (n = 8 replicate wells). These experiments have been performed independently more than three times (Suppl. Figs. S1B,C and S5A,C also show the performance of control molecules in biological replicates).