Table 3.
Statistical evaluation of PCR-ARMS, PCR-HRM, and direct sequencing to PCR-RFLP.
| PCR-ARMS | PCR-HRM | Direct Sequencing | |
|---|---|---|---|
| Positive predictive value (%) | 100 | 100 | 100 |
| Positive predictive value 95%IC | 92.6–100 | 92.6–100 | 92.4–100 |
| Sensitivity (%) | 96 | 96 | 94 |
| Negative predictive value (%) | 97.7 | 97.7 | 96.6 |
| Negative predictive value 95%IC | 92–99.4 | 92 –99.4 | 90.5–98.8 |
| Specificity (%) | 100 | 100 | 100 |
| Accuracy (%) | 98.5 | 98.5 | 97.8 |
| Concordance (%) | 96.8 | 96.8 | 95.2 |
| Concordance 95%IC | 95.6–97.7 | 95.6–97.7 | 93.3–96.5 |
| Bias correction factor Cb (% accuracy) | 99.9 | 99.9 | 99.9 |
| Weighted Kappa | 0.97 | 0.97 | 0.95 |
| Weighted Kappa 95%IC | 92.4–100 | 92.4–100 | 89.8 to 100 |