Table 4.
Effect of the addition of blocking agents on the results of the Beckman Coulter Access® Beckman Coulter hormone assays suspected of interference by endogenous antibodies.
| Assay | Blocker |
Patient sample |
QC sample |
||
|---|---|---|---|---|---|
| Result | Recovery (%) | Result | Recovery (%) | ||
| TSH (mUI/mL) | Untreated | 12.86 | – | 26.19 | – |
| +Pool 1 | 0.61 | 4.7 | 28.63 | 109.3 | |
| +Pool 2 | 1.07 | 8.3 | 30.13 | 115.0 | |
| LH (mUI/mL) | Untreated | 121.80 | – | 66.54 | – |
| +Pool 1 | 23.42 | 19.2 | 51.43 | 77.3 | |
| +Pool 2 | 21.49 | 17.6 | 61.67 | 92.7 | |
| FSH (µUI/mL) | Untreated | 47.46 | – | 42.57 | – |
| +Pool 1 | 7.96 | 16.8 | 34.44 | 80.9 | |
| +Pool 2 | 6.61 | 13.9 | 39.80 | 93.5 | |
| Prolactin (ng/mL) | Untreated | 39.87 | – | 43.17 | – |
| +Pool 1 | 8.24 | 20.7 | 40.13 | 92.9 | |
| +Pool 2 | 11.55 | 29.0 | 41.68 | 96.6 | |
Pool 1 and pool 2 were mixtures of different blockers: PolyMAK-33, HBR-1 and goat, mouse, rabbit and bovine immunoglobulins (see text). The manufacturer did not reveal the exact composition of each of the pools.