FIG 1 .
Phylogenetic placement of the genomes used in this study within the acI lineage. The tree was built using RAxML (41) from a concatenated alignment of protein sequences from 37 single-copy marker genes (40). The order Actinomycetales forms the outgroup. Vertical black bars indicate groups of genomes belonging to defined tribes/clades within the acI lineage, as determined using 16S rRNA gene sequences (for SAGs and bin FNEF8-2 bin_7 acI-B only) and a defined taxonomy (28). SAGs are indicated with italic text. Figure S1 shows the position of the acI lineage relative to other orders within the class Actinobacteria.