TABLE 2 .
Susceptibility of E. coli TOP10 transformants harboring chromosomal fosA from various species
| Transformant | MIC (μg/ml)a | Zone diam (mm) | Zone diam with PPF (mm) | Original species | Accession number |
|---|---|---|---|---|---|
| E. coli TOP10 carrying plasmid | |||||
| pFosAKP | >1,024 | 6 | 18 | K. pneumoniae | YP_005224903.1 |
| pFosAEC | >1,024 | 6 | 20 | E. cloacae | AIX57742.1 |
| pFosAEA | >1,024 | 6 | 16 | E. aerogenes | YP_004592226.1 |
| pFosASM | 1,024 | 6 | 14 | S. marcescens | WP_025303168.1 |
| pFosAPA | 16 | 22 | 28 | P. aeruginosa | NP_249820.1 |
| pFosAMM | 1,024 | 6 | 16 | M. morganii | WP_004238530.1 |
| pFosAKO | 1,024 | 6 | 20 | K. oxytoca | WP_047724618.1 |
| pFosAPS | >1,024 | 6 | 18 | P. stuartii | WP_014658192.1 |
| E. coli TOP10 alone | 1 | 38 | 38 |
a
MICs were determined by the agar dilution method supplemented with 25 μg/ml glucose-6-phosphate. For disk testing, 1 mg of sodium phosphonoformate (PPF) was added to fosfomycin disks. The fosA genes were cloned and constitutively expressed on vector pUC57.