FIG 1 .

clsA is the major cardiolipin synthase of S. flexneri. (A) Schematic of E. coli cardiolipin synthesis (30–32). The chemical structures of phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and cardiolipin (CL) were produced using ChemDraw (PerkinElmer). (B) TLC analysis of total S. flexneri membrane phospholipids of the wild type (WT) and cardiolipin synthesis mutants. Bacteria were grown to mid-log phase, and phospholipids were extracted and separated by TLC. The percentage of cardiolipin in the sample is indicated above each lane. pclsA, plasmid expressing clsA. (C) Bacteria were grown into stationary phase (OD₆₅₀ of ~2.0); phospholipids were then extracted and separated by TLC. The percentage of cardiolipin in the sample is indicated above each lane. Phospholipids were visualized using molybdenum blue spray reagent (Sigma).