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Purification of recombinant CRP expressed in P. pastoris X-33. After 48 h of constitutive expression in optimum medium, the recombinant CRP was purified from the culture supernatant by HisTrap HP-affinity column chromatography. a The purified recombinant CRP was detected via SDS-PAGE analysis. b The purified recombinant CRP was confirmed via western blot analysis. Lane M protein marker. Lane 1 culture supernatant prior to purification. Lane 2 eluate fraction from affinity chromatography
