Table 2.
Recovered versus predicted functions of climate adaptation
| A Predicted functions of thermal adaptation genes | B Candidate genes associated with predicted functions | C Outlier loci from A. cybotes; and their interactants |
|---|---|---|
| 1 Lipoprotein/lipid metabolism1,2, Lipid transport2, Body mass | LPL1, CD361,6,7,8, CETP1, MAPK11, MAPK141, SOD11, STUB11, LEPR1, FABP38, UCP31 | CETP, LPL, MAPK14, (LEP), (APOB), SOX9, CALCR, MROH7, (FABP7), (FABP1), FABP2,(LPA/APOA) |
| 2 Solute carrier Membrane channels controlling water loss/retention or cryoprotectant1 | LPL1, CETP1 | LPL, CETP, MFSD6 |
| 3 Stress response and homeostasis1,2 Incl. heat stress1 | Heat shock proteins; HSP70/HSPA1A1,9,10, HSPA41, HSPA51, PON11,7, MAPK11, UCP31, HSF11, MAPK141, HSP471, UNG1, HSPB21, SOD11, STUB11, HSPA81 |
MAPK14, GLTSCR2, HSPA8, (HSP90AA1); HSP70/HSPA1A, HSPA4 HSPA5, PON1, (PON2) SOX9, UBE4B |
| 4 Phenotypic or Phenological changes related to thermal adaptation1, Developmental Process2 | Not overrepresented1 | MC1R, MC2R, MC3R, MC4R, MC5R, POMC, CALCR, SOX9, (ADORA2A), SPTBN1 11 |
| 5 Cell signal relay1 | MAPK11, UCP31, HSF11, ADORA2B1, ADRB21, MAPK141, HSP471, UNG1, HSPB21, MC4R1, SOD11, STUB11, HSP481, EGFR1, CD361,6,7,8, MRAS1, ADORA11,8 | ADRB2, ADORA2B, MAPK14, MRAS, MC4R, GLTSCR2, RASGRP3, RAP1GAP2 |
|
6 Levels of Oxygen response, Oxidative stress response, hypoxia1,3
Oxidation‐reduction process2 |
UCP31, SOD11 | (FABP1), (HSP90B1), (LEP), (SOD2), (VEGFA), GLTSCR2 4, (HSP90AA1), SIRT6 |
| 7 Muscle contraction and relaxation, muscle development1 | ADORA2B1, SOD11, HSPB21 | ADORA2B, DYNC1H1 |
| 8 Vasodilation1, blood circulation1, blood pressure regulation1, endothelial function1 | ADORA2B1, SOD11, POMC1 | ADORA2B, POMC, (ADRB1), RAP1GAP2, RASGRP3, (VEGFA), (HPSE), SPTBN1 |
|
9 Regulation of Gene Expression2
incl.: Chromatin modification, Transcription2, RNA metabolic process2, RNA processing2, Translational Regulation, regulation of elongation2, Protein folding2, Proteolysis involved in cellular protein catabolic process2 |
No genes predicted1 | ASF1B, SIRT6, PHF20, UBE4B, SOX9 |
| 10 Cellular component biogenesis2 | No genes predicted1 | ADORA2A, ADORA2B, ADRB1, ADRB2, ADRB3, CALCR, MC1R, MC2R, MC3R, MC4R, MC5R |
| D Additional functions, not previously predicted | ||
| 11 Aging and DNA repair | No genes predicted1, CALCR 6 | CALCR, SIRT6, SOD2 |
| 12 Other, unknown | LRBA, MFSD6, MROH7 |
A—Predicted functions of genes underlying climate adaptation. B—Thermal adaptation candidate gene list. C—Genes verified in Anolis cybotes interactome. In bold: outlier loci; In regular: predicted candidate genes; In parentheses: novel genes with similar function as predicted genes. Table References (superscript numbers): (1) Wollenberg Valero et al., 2014; (2) Porcelli et al., 2015; (3) Yang et al., 2014; (4) Kim, Park, & Lee, 2012; (5) Chen et al., 2010; (6) Hancock et al., 2008; (7) Vermillion, Anderson, Hampton, & Andrews, 2015; (8) Holsinger, Schultz, & Hightower, 1996; (9) Sørensen, Dahlgaard, & Loeschcke, 2001; (10) Estrada et al., 2012.