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. 2017 Aug 25;8:1044. doi: 10.3389/fimmu.2017.01044

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Copyright © 2017 Braun, Python and Summerfield.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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B cell subsets in peripheral blood of pigs. (A) Gating strategies for identifying B cell subsets based on expression of IgG, IgM, CD21, and CD11R1. After doublet exclusion, population 1 (P1) was defined as CD21⁺IgM⁺, P2 as IgG⁺, P3 as CD21⁻IgM⁺CD11R1⁺, and P4 as CD21⁻IgM⁺CD11R1⁻. (B) Histogram overlays of FSC, IgM, CD11c, CD80/86, CD14, phosphor-PLC-γ2, CD115, and CD172a for the subsets P1–P4 identified in (A). A representative animal out of three is shown.