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. 2017 Aug 29;7:9582. doi: 10.1038/s41598-017-09543-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of direct electric current on the cell viability. The LIVE/DEAD assay images of (a) ADSC under 0 µA, (b) ADSC under 1000 µA for 3 h, (c) hMSC under 0 µA, (d) hMSC under 1000 µA for 3 h, (e) TMSC under 0 µA, (f) TMSC under 1000 µA for 3 h (live cells: green, Calcein AM/dead cells: red, EthD-1). Scale bar = 100 μm. The MTT assay of (g) ADSC, (h) hMSC, (i) TMSC for 1, 3d culture after 0 or 1000 µA treatment for 3 h.