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. 2017 Aug 29;7:9582. doi: 10.1038/s41598-017-09543-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mixture of TMSC and osteogenic differentiated cells at different OsD days. The immunofluorescence images of TMSC and osteogenic differentiated cells mixture ratio of (a) 7:3, (b) 5:5, (c) 3:7 at day 3, (d) 7:3, (e) 5:5, (f) 3:7 at day 7, and (g) 7:3, (h) 5:5, (i) 3:7 at day 14. The histogram of TMSC and osteogenic differentiated cell mixture using electrotaxis analysis and Youden index under 1000 µA at (g) day 3, (h) day 7, (i) day 14. Cut point of TMSC vs. osteogenic differentiated cells was 22.42 μm/h. Scale bar = 100 μm.