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. 2017 Aug 29;7:9611. doi: 10.1038/s41598-017-10082-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of HEDTA and temperature on the nucleation and growth of fluorapatite (a) shown in the XRD patterns of precipitates from the HEDTA solution (blue line) and the non-HEDTA group (yellow line). (b) TEM of the precipitate from the HEDTA solution, which showed PNCs of approximately 5 nm aggregating around ACP. (c,d) Time course of the amount of free calcium ions measured by a calcium ion-selective electrode (Ca-ISE) in different phosphate solutions at different temperatures.