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. 2017 Aug 29;7:9703. doi: 10.1038/s41598-017-10309-x

Figure 5.

Figure 5

Preferential activation of iNKT cells among liver MNCs following stimulation with α-mannosyl glycolipids. Liver MNCs prepared from wild-type mice were cultured in the presence of α-mannosyl glycolipids. After two days cells were triply stained with fluorescence-labeled anti-CD69 antibodies, anti-TCR Cβ antibodies and CD1/α-GalCer tetramers, and CD69 expression in both iNKT cell (TCR Cβ+, CD1/α-GalCer tetramer+) and conventional T cell populations (TCR Cβ+, CD1/α-GalCer tetramer) was analyzed by flow cytometry.