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. 2017 Aug 29;7:9604. doi: 10.1038/s41598-017-07639-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of the major bacterial phyla and classes along the toposequence and hierarchical clustering analysis. (A) Taxonomic distribution. The relative abundance of the major phyla and classes was calculated as the percentage of sequences belonging to a particular lineage of all 16S rRNA gene sequences recovered from each sample. The Proteobacteria phylum is represented in green and was detailed for the different classes. The Actinobacteria phylum was represented in blue and was detailed for the Actinomycetales and Solirubrobacterales orders. (B) Hierarchical clustering analysis. A hierarchical clustering based on Bray-Curtis (BC) distance of the major bacterial phyla and classes was performed. Samples are referred as follow: Calcaric.BS: Calcaric bulk soil; Calcaric.Rh: Calcaric rhizosphere; Eutric.BS: Eutric bulk soil; Eutric.Rh: Eutric rhizosphere; Hyperdystric.BS: Hyperdystric bulk soil; Hyperdystric.Rh: Hyperdystric rhizosphere.