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. 2017 Aug 29;7:9674. doi: 10.1038/s41598-017-10013-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impaired recruitment of Rad51 to damage sites in Ape1-depleted cells, and its restoration by simultaneous depletion of Chk2. (A) siRNA-treated U251-MG cells were exposed to IR (3 Gy), fixed at the indicated time points, and immunostained for Rad51. Scale bars, 5 µm. (B) Rad51 foci were quantified as described in Fig. 4. *p < 0.05; (siAPE1#3 vs. siAPE1#3/siCHEK2); Student’s t test.