Table 3.

Characteristics of CA from some pathogenic bacteria.

Sl. No.	Organism	Type	Cloning Vector/Host	Molecular weight	Other Features	References
1	V. cholerae	α	E. coli (pET15b)	26.4 kDa	Km = 11.7 mM kcat = 8.23 × 105s−1 kcat/KM = 7.0 × 107 M−1 s −1	Del Prete et al., 2012
2	Brucella suis	B	E. coli (pET15b)	25 kDa	kcat = 1.1 × 106, kcat/Km = 8.9 × 107 M−1 s−1	Joseph et al., 2010
3	H. pylori	α	E. coli (pACA1)	28.28 kDA	kcat = 2.4 × 105s−1 KM = 17 mM kcat/KM = 1.4 × 107 M−1s−1 at pH 8.9 and 25°C	Chirica et al., 2001
4	Salmonella typhimurium	β (two stCA1 and stCA2)	E. coli (pGEX-4T2)	24.8 kDa (stCA1) 26.6 kDa (stCA2)	kcat = 0.79 × 106 s−1 and 1.0 × 106 s-−1, kcat/Km = 5.2 x107M−1s−1 and 8.3 × 107 M−1 s−1	Vullo et al., 2011
5	Mycobacterium tuberculosis	β (mtCA1)	E. coli pGEX-4T2	NA	kcat = 3.9 × 105 s−1 kcat/ Km = 3.7 × 107 M−1 s−1	Minakuchi et al., 2009
6	Streptococcus pneumoniae	β (PCA)	E. coli	NA	kcat = 7.4 × 105 s−1 kcat/Km = 6.5 × 107 M−1 s−1 at an optimum pH of 8.4	Burghout et al., 2011
7	Plasmodium falciparum	η (PfCA)	E. coli	NA	kcat = 1.4 × 105 s−1kcat/Km = 5.4 × 106 M−1s−1	Del Prete et al., 2014
8	Haemophilus influenzae	β (HICA)	E. coli	105.26 kDA (native molecular weight)	Tetramer, presence of a novel non-catalytic bicarbonate binding site, kcat, kcat/Km is pH dependent	Cronk et al., 2006
9	Candida albicans	β (Nce103)		31.5 Da	kcat = 8.0 × 105 s−1kcat/Km = 9.7 × 107 M−1s−1	Innocenti et al., 2009a
10	Malassezia globosa	β (MG-CA)	E. coli	27 kDa	kcat = 8.6 × 105 s−1kcat/Km = 6.9 × 107 M−1s−1 Ki (acetazolamide) 76000 ± 450 nm	Hewitson et al., 2012
11	Cryptococcus neoformans	β (Can2)	E. coli	26 kDa	kcat = 3.9 × 105 s−1kcat/Km 4.3 × 107 M−1s−1Ki (acetazolamide) 10.5 nm (homodimer)	Mogensen et al., 2006; Innocenti et al., 2009a
12	Aspergillus fumigatus	4-βCAs (cafA-cafD)	Saccharomyces cerevisiae	–	All four CAs strongly expressed during pathogensis	Han et al., 2010; Tobal and Balieiro, 2014