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. 2017 Aug 29;7:9677. doi: 10.1038/s41598-017-09814-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of advanced maternal age placental and arterial function. (a) Placental system A activity (Na⁺-dependent ¹⁴C-MeAIB uptake), (b) placental TauT activity (Na⁺-dependent ³H-TauT uptake). Mean ± SEM; linear regression (difference in intercept and elevation, *p < 0.05, **p < 0.005). (c) Chorionic plate artery constriction in response to U46619 and (d) relaxation in response to sodium nitroprusside (SNP) in pre-constricted CPA (n = 15/age group). (e) Myometrial artery constriction to U46619, and (f) relaxation to bradykinin (BK) in myometrial vessels from women ages 20–30 (n = 9), 35–39 (n = 17) and ≥40 (n = 6) years of age. Mean ± SEM, 2-Way ANOVA with Bonferroni’s post hoc test, *p < 0.05, **p < 0.01.