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. 2017 Aug 29;7:9677. doi: 10.1038/s41598-017-09814-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fetal and placental measurements from control and advanced maternal age (AMA) mouse pregnancies. (a) Fetal weights, (b) fetal weight frequency distribution, (c) placental weights, (d) placenta weight frequency distribution, (e) fetal:placental weight ratios and (f) fetal:placental weight ratios frequency distribution. Data in (a,c and e) are presented as litter averages [N = 22 for controls (n = 159 individual fetuses), N = 13 for AMA viable (n = 99 individual fetuses), N = 12 for AMA non-viable (n = 14 individual fetuses)]. *p < 0.05, **p < 0.01, ***p < 0.001, Kruskal-Wallis with Dunn’s Multiple Comparison Test. For fetal weight frequency distribution (b) (control = black line, AMA = red line), the dotted line refers to 5^th centile of control population. For placental weight frequency distribution (d) the dotted line refers to 95^th centile of control population. For FW:PW ratios frequency distribution (f) (control = black line, AMA Viable = red line, AMA Non-Viable = blue line) the dotted line refers or 2.5 standard deviations (SD) away from the mean of control population.