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. 2017 Aug 29;7:9677. doi: 10.1038/s41598-017-09814-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of maternal age on AMA murine amino acid transfer and umbilical and uterine artery function. (a) Rate of uni-directional maternofetal clearance of ¹⁴C-MeAIB per gram of placenta (µl/min/g placenta), (b) total maternofetal transfer of ¹⁴C-MeAIB independent of fetal or placental size, (c) the rate of uni-directional maternofetal clearance of ³H-Taurine per gram of placenta (µl/min/g placenta), and (d) total maternofetal transfer of ³H-Taurine independent of fetal or placental size **p < 0.001, Mann-Whitey U Test. Effect of advanced maternal age on umbilical artery: (e) constriction to U46619, and (f) relaxation to sodium nitroprusside (SNP) (control n = 6, AMA n = 4) and uterine artery: (g) constriction to a phenylephrine and (h) relaxation to acetyl choline (ACh). Data are mean ± SEM. 2 Way ANOVAs with Bonferroni’s Multiple Comparison (con n = 7, AMA n = 5), *p < 0.05.