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. 2017 Aug 25;10:270. doi: 10.3389/fnmol.2017.00270

Figure 2.

Figure 2

PDK1 is localized in the nerve terminals of the neuromuscular junction (NMJ). (A,B) Double staining labeled PDK1 (in green) and AChRs (fluorescent α-BTX in red). The images show PDK1-positive green immunolabeling concentrated at the presynaptic area over the red postsynaptic gutters. (C) Triple staining labeled PDK1 (in green), AChRs (in red) and nerve terminal (with anti-syntaxin antibody in blue, Syntx). The fine granulated label for PDK1 was well colocalized with syntaxin in the presynaptic nerve terminal position (over the AChRs-positive postsynaptic red gutters). (D) Triple staining labeled PDK1 (in green), AChRs (in red) and Schwann cell (with anti-S100 antibody in blue) showed that PDK1 was not colocalized with Schwann cell (see arrows). Thus, indicating that PDK1 is exclusively located at the presynaptic component of NMJ (n = 5; 25–30 endplates per muscle). The scale bars indicate 10 μm. Abbreviations: PDK1, phosphoinositide-dependent kinase 1; AChRs, Acetylcholine receptors; Syntx, syntaxin; S100, S100 protein; α-BTX, α-bungarotoxin.