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. 2017 Aug 25;10:270. doi: 10.3389/fnmol.2017.00270

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Copyright © 2017 Hurtado, Cilleros, Just, Simó, Nadal, Tomàs, Garcia, Lanuza and Tomàs.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Total PDK1 levels and its phosphorylation are unaltered after synaptic activity and muscle contraction. PDK1 and pPDK1 in presynaptic stimulation treatment, Contraction treatment and Presynaptic stimulation with contraction treatment at 1 Hz stimulation for 30 min. Presynaptic stimulation has been simplified as Stimulation. Each column has been compared to its respective control (see Table 1). Results showed that PDK1 and pPDK1 levels, as well as pPDK1/PDK1 ratio, remained unaltered after nerve stimulation with and without muscle contraction. Data are mean percentage ± SEM, (Stimulation n = 7, Contraction n = 6 and Stimulation with Contraction n = 6). Abbreviations: PDK1, phosphoinositide-dependent kinase 1; pPDK1, phosphorylated phosphoinositide-dependent kinase 1.