Figure 2.

A: Treatment procedure of the bilateral subcutaneous melanoma model. DBA/2 mice were subcutaneously inoculated with 1×10⁶ clone M3 cells in their bilateral flanks. When tumors reached approximately 3-4 mm in maximal diameter (day 0), mice were randomly divided into six treatment groups: mock (group 1), HF10 (group 2), DTIC1 and 2 (groups 3 and 4, respectively) and HF10+DTIC1 and 2 (groups 5 and 6, respectively). i.p.; intraperitoneal injection, i.t.; intratumoral injection. B: Tumor growth inhibition by HF10 local injection and/or DTIC intraperitoneal injection. Subcutaneous tumor were treated with HF10 and/or DTIC. Volume of the treated tumor (right flank) or the untreated tumor (left flank) was measured for the indicated days after treatment. C: Survival analysis of mice after treatment. HF10-DTIC combination therapy showed a significant prolonged survival effect compared to the control group (P = 0.004).