Figure 6.

Treatment with Tamoxifen and a PI3K Inhibitor Controls MCF-7 Tumor Growth In Vivo. Female Balb/c nude mice were inoculated with MCF-7 cells at the flank and randomized into six different treatment groups when the tumor volume reached 500 mm³. (A) Tumor volumes measured using a caliper for treatments with CTL (solid circles; PBS), T (open circles; tamoxifen, 50 mg/kg), BYL (solid triangles; alpelisib, 35 mg/kg), T + BYL (open triangles; tamoxifen + alpelisib, 50 mg/kg + 35 mg/kg), BKM (solid squares; 25 mg/kg), and T + BKM (open squares; tamoxifen + buparlisib, 50 mg/kg + 25 mg/kg). The figure is representative of two independent experiments (N = 5 and 7 in each treatment group). (B) Representative figures of paraffin-embedded MCF-7 tumors from (1) CTL, (2) T, (3) BYL, (4) T + BYL, (5) BKM, and (6) T + BKM treatments were stained for pAKT, pS6, and p-p70S6K. All images were obtained using a 10x eyepiece and 40x objective lens (scale bar = 50 µm).