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. 2017 Aug 29;7:9706. doi: 10.1038/s41598-017-10341-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Response of ex vivo mouse mesenteric arteries to transfection with RV-BPIFB4. (A,B) Graphs of vascular response to increasing doses of acetylcholine (ACh) or nitroglycerine in mouse mesenteric arteries before and after transfection with plasmids encoding WT-BPIFB4. (C,D) Graphs of vascular response to increasing doses of acetylcholine (ACh) or nitroglycerine in mouse mesenteric arteries before and after transfection with plasmids encoding RV-BPIFB4 (n = 7 for each experiment). *p < 0.05; **p < 0.01;***p < 0.001 (two-way ANOVA followed by Bonferroni post hoc test). (E) left, Representative immunoblot of mesenteric artery lysate collected after vascular reactivity studies; right, Optical density measurements of immunoblottings. Columns are the mean ± SEM of 3 independent experiments. *p < 0.05 (one-way ANOVA analysis followed by Bonferroni post hoc test). Images were cropped using Adobe Photoshop, full-length blots are presented in Supplementary Fig. 2.