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. 2017 Aug 29;7:9875. doi: 10.1038/s41598-017-10113-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sucrose response scores are similar following repeated exposures. Median, quartiles and max and min (upper and lower whiskers) SRS values of bees evaluated after a first and second exposure to mineral oil or to one of the three pheromone components (geraniol, IPA and 2H). Red dots represent individual bees. For each bee, SRS was established by measuring PER to a series of six sucrose solutions of increasing concentration (from 0.1% to 30%). SRS values ranged between six (bees responding to all 6 concentrations) and 0 (bees not responding to any concentration). For each of the two sessions, non-responding bees (i.e. bees not responding even to an additional concentration of 50%) were discarded as their SRS value could not be established. In all groups, the SRS remained invariable between the two successive exposures (Wilcoxon test, oil: n = 84, p = 0.18; geraniol: n = 70, p = 0.96; IPA: n = 55, p = 0.98. 2H: n = 32, p = 0.27).