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The Staf DNA binding domain can repress transcriptional activation of a U6 gene carrying a compact promoter. Identities of the effector proteins and reporter genes are indicated above each lane. Lanes 1 and 5, no effector was expressed. Lanes 2–4 and 6, cytoplasmic expression of variable amounts of mRNA effectors: lane 2, 0.1 ng; lane 3, 1 ng; lanes 4 and 6, 5 ng. Lanes 1–6, oocyte nuclei microinjected with 0.5 ng of the indicated reporter.
