Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 29;7:9773. doi: 10.1038/s41598-017-09936-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Estimation of cellular cofactors (NADPH and NADPt) and the involved enzymes glucose-6-phosphate dehydrogenase (G6PD) and glucose-6-phosphat-isomerase (GPI). (A) Schematic representation of enzymes involved in redirection of the flux from glycolysis to Pentose Phosphate Pathway (PPP). The rerouting of glucose into oxidative PPP depends on a rapid increase of the G6PD flux, which can generate strong inhibitor of GPI thus facilitating a forward flux into PPP. (B) Analysis of G6PD and GPI enzyme activity: The enzyme assay was carried out in a 96-well plate in cells exposed to B[a]P (0.5 µM, 24 h). NADPH (100 µM) was used as an inhibitor for G6PD, while 6-PG (5 mM) was used as an inhibitor for GPI enzyme assay. Effects of 0.5 µM B[a]P are shown on cellular (C,D) and cytosolic (E,F) NADPH/NADP⁺ ratio and NADPH content of RT4 cells. The data is presented as mean ± standard error of the mean of four independent experiments. The level of significance relative to the control was determined by using the Anova test (*p < 0.05, ***p < 0.001).