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. 2017 Aug 29;7:9580. doi: 10.1038/s41598-017-10057-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Knockdown of Dm eIF5A in the embryonic epidermis produces defects in DC and actin cable assembly. (A) Schematic representation of the anterior-posterior (AP) compartment boundary of a Drosophila wing imaginal disc (shaded in pink). (B) Anti-eIF5A antibody staining of a dpp-GAL4/UAS-iReIF5A wing imaginal disc, in which eIF5A knockdown is induced in the AP boundary. (C,D) Dark-field micrographs of representative (C) wild-type and (D) eIF5A mutant embryos (eIF5A ^KD). Anterior is to the left, dorsal is up. White arrowheads point to holes or not properly sealed areas in the anterior and dorsal parts of the embryo. (E,F) Confocal images of the lateral epidermis, DME cells and AS cells of representative stage 13–14 (E) control and (F) eIF5A ^KD embryos stained with phalloidin. Blue arrows point to the actomyosin cable in DME cells, which is irregular and discontinuous in eIF5A mutants. Detachment of DME cells from AS cells is frequently observed in mutant embryos (red arrowheads in F). Scale bars: 100 µm in C,D; 25 µm in E,F.