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. 2017 Aug 29;7:9783. doi: 10.1038/s41598-017-10004-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of different methods for extracting Z-ligustilide from A. Sinensis. In here, RAS powder was pretreat with different cellulase for 30 h at 37 °C, and then reflux extraction was performed and the concentration of Z-ligustilide was determined by HPLC. Furthermore, the numbers 1102, 1201 and 1301 represents the three identified endophyte bacterial strains: No.Lut1102, No.Lut1201 and No.Lut1301, respectively. E corresponds to the respective cellulase produced by the bacteria. IE represents the inactivated enzymes of the corresponding reference; blank was without any enzyme treatment using the traditional extraction method. Cellulase represents commercially available cellulase assisted extraction of Z-ligustilide ^*,&,#p < 0.05 compared with commercially available cellulases, blank and inactivate enzyme control groups.