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. 2017 Aug 29;7:9585. doi: 10.1038/s41598-017-10049-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transfection efficiency and cytotoxicity of PSL and PS complexes in HeLa cells at various N/P ratios in the presence of 10% FCS. Transfection was performed at a dose of 0.3 μg of DNA per well in a total volume of 200 μl. GFP expression was quantified by flow cytometry after 24 hours. (a) Percentage of GFP-positive HeLa cells. (b) Geometric mean of fluorescence intensity of GFP-positive cells. (c) Cell viability assay of HeLa cells after transfection. Data represent mean ± standard deviation of 3 independent experiments.