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. 2017 Aug 29;7:9585. doi: 10.1038/s41598-017-10049-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transfection and cellular uptake of PS and PSL complexes in fetal calf serum (FCS) conditions. Transfection and cellular uptake studies were performed at a dose of 0.3 μg of DNA per well of 48-well plate. (a) Effect of serum on the transfection efficiency of PS and PSL complexes. Transfection was performed at various serum concentrations. (b) Mean fluorescence intensity of GFP-positive HeLa cells was measured by flow cytometry 24 h after transfection at various serum concentrations. (c) Cellular uptake of PS and PSL complexes as a function of serum concentrations. (d) Geometric mean of fluorescence intensity (Geo mean) of fluorescent cells as a function of serum concentration. Data represent mean ± standard deviation of 3 independent experiments.